Genetic Differentiation of Red Clover (Trifolium pratense L.) Cultivars and Their Wild Relatives

Biodiversity of red clover is changing. It become more difficult to find new breeding material. Moreover, populations with predominant morphological features cultivars are frequently found in wild habitats. Therefore, it important identify genetic structure and diversity within among worldwide. During this research, seed samples from (Trifolium pratense L.) habitats were collected different Lithuanian sites nemoral environmental zone. Subsequently, germinated greenhouse randomly taken for analyses. ISSR fingerprints 339 genotypes 13 5 varieties analysed. Analysis molecular variance (AMOVA) has shown high variation (83%). The highest polymorphism was identified population pop2887 (PPL = 82.28%). Interestingly the ranged 64.56 (‘Vytis’) 83.54 (‘Liepsna’). Meanwhile, low—17%. Fixation index FST 0.182 revealed differentiation while gene flow an exchange 2.2474 migrants per generation. This a between Lithuania.